In the St. Croix Lab, we develop and utilize a combination of advanced optical imaging technologies to dissect molecular signaling pathways controlling vascular function in rodent and zebrafish model systems of disease. Much of this work involves the application of optical microscopy to the study of living systems, specifically combining free radical imaging, FRET, confocal, multiphoton and physiologic measurements in live model systems, tissues and cells.
In my leadership role at the CBI, I have well-established, active and productive collaborations with NIH funded investigators that use an array of elite level imaging modalities to study molecular signaling events in living cells, tissue and animal models. Specifically, one of my major roles within the CBI is to manage and direct the use and application of fluorescence-based optical microscopy for collaborators within the University of Pittsburgh School of Medicine. This includes the design and development of appropriate imaging and image analysis approaches with faculty; overseeing training; managing access; and ensuring the devices are well maintained and managed by CBI staff members.
Links to publications: https://www.ncbi.nlm.nih.gov/myncbi/browse/collection/46336677/?sort=date&direction=ascending
- Wenzel SE, Tyurina YY, Zhao J, St Croix CM, Dar HH, Mao G, Tyurin VA, Anthonymuthu TS, Kapralov AA, Amoscato AA, Mikulska-Ruminska K, Shrivastava IH, Kenny EM, Yang Q, Rosenbaum JC, Sparvero LJ, Emlet DR, Wen X, Minami Y, Qu F, Watkins SC, Holman TR, VanDemark AP, Kellum JA, Bahar I, Bayır H, Kagan VE. PEBP1 Wardens Ferroptosis by Enabling Lipoxygenase Generation of Lipid Death Signals.. Cell. 2017 Oct 19;171(3):628-641.e26. doi: 10.1016/j.cell.2017.09.044. PubMed PMID: 29053969;
- Kagan VE, Mao G, Qu F, Angeli JP, Doll S, Croix CS, Dar HH, Liu B, Tyurin VA, Ritov VB, Kapralov AA, Amoscato AA, Jiang J, Anthonymuthu T, Mohammadyani D, Yang Q, Proneth B, Klein-Seetharaman J, Watkins S, Bahar I, Greenberger J, Mallampalli RK, Stockwell BR, Tyurina YY, Conrad M, Bayır H. Oxidized arachidonic and adrenic PEs navigate cells to ferroptosis.. Nat Chem Biol. 2017 Jan;13(1):81-90. doi: 10.1038/nchembio.2238. PubMed PMID: 27842066;
- St Croix CM, Steinhorn RH. New Thoughts about the Origin of Plexiform Lesions.. Am J Respir Crit Care Med. 2016 Mar 1;193(5):484-5. doi: 10.1164/rccm.201510-1959ED. PubMed PMID: 26930432;
- He J, Wang Y, Missinato MA, Onuoha E, Perkins LA, Watkins SC, St Croix CM, Tsang M, Bruchez MP. A genetically targetable near-infrared photosensitizer.. Nat Methods. 2016 Mar;13(3):263-8. doi: 10.1038/nmeth.3735. PubMed PMID: 26808669;