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Guang Li Ph.D.

  • Assistant Professor- Organoid, iPSC, Single cell sequencing, Heart development
Research Interests

Heart development as a critical embryonic developmental process is tightly regulated on the cellular and molecular level. If this process goes awry, it will lead to congenital heart diseases (CHD), which accounts for a significant portion of stillbirths and is present in 1-2% of all live births. The Li lab is using advanced techniques including human induced pluripotent stem cells (hiPSC), single-cell RNA sequencing, single molecular in situ hybridization, CRISPR/Cas9, and tissue cleaning methods to decode the spatial and temporal information of each single cardiac cells. Meanwhile, with the knowledge gained from the study of basic cardiac lineage regulations, the lab is also exploring the lineage defects in congenital heart diseases using patient-derived iPSCs.              

Representative Publications
  1. Wei Feng, Lyuqin Chen, Patricia K. Nguyen, Sean M. Wu, Guang Li (2019) Single cell analysis of endothelial cells identified organ-specific molecular signatures and heart-specific cell populations and molecular features. Frontiers in Cardiovascular Medicine. In Press. 
  2. Wei Feng, Andrew Przysinda, Guang Li (2019) Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells. JoVE. In Press. 
  3. Li G.,* Tian L., Goodyer W., Kort E., Buikema J., Xu A., Wu J., Jovinge S. *, Wu SM* (2019) Single cell expression analysis reveals anatomical and cell cycle-dependent transcriptional shifts during heart development. Development. PMID: 31142541 *Corresponding author. 
  4. Goodyer WR, Beyersdorf BM, Paik DT, Tian L, Li G, Buikema JW, Chirikian O, Choi S, Venkatraman S, Adams EL, Tessier-Lavigne M, Wu JC, Wu SM. (2019) Transcriptomic Profiling of the Developing Cardiac Conduction System at Single-Cell Resolution. Circulation Research. PMID: 31284824. 
  5. Tabula Muris Consortium; Overall coordination; Logistical coordination; Organ collection and processing; Library preparation and sequencing; Computational data analysis; Cell type annotation; Writing group; Supplemental text writing group; Principal investigators. (2018) Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris. Nature. PMID: 30283141.
  6. Su T., Stanley G., Sinha R., D’Amato G., Das S., Rhee S., Chang AH., Poduri A., Raftrey B., Dinh TT., Roper WA., Li G., Quinn KE., Caron KM., Wu SM., Miquerol L., Butcher EC, Weissman I., Quake S., Red-Horse K (2018) Single-cell analysis of early progenitor cells that build coronary arteries. Nature. PMID: 29973725
  7. Li G., Dzilic E., Flores N., Shieh A., Wu S.M. (2017) Strategies for the acquisition of transcriptional and epigenetic information in single cells. Journal of Thoracic Disease. PMID: 28446964.
  8. Doppler SA, Deutsch MA, Serpooshan V, Li G., Dzilic E, Lange R, Krane M, Wu S.M. (2017) Mammalian Heart Regeneration: The Race to the Finish Line. Circulation Research. PMID: 28209796.
  9. Chuang W, Sharma A, Shukla P, Li G., Mall M, Rajarajan K, Abilez O.J., Hamaguchi R, Wu J.C., Wernig M, Wu S.M. (2017) Partial Reprogramming of Pluripotent Stem Cell-Derived Cardiomyocytes into Neurons. Scientific Reports. PMID: 28327614.
  10. Gregoire S, Li G., Sturzu AC, Schwartz RJ, Wu S.M. (2017) YY1 Expression Is Sufficient for the Maintenance of Cardiac Progenitor Cell State. Stem Cells. PMID: 28580685
  11. Li D, Liu J, Liu W, Li G., Yang Z, Qin P, Xu L. (2017) The ISWI remodeler in plants: protein complexes, biochemical functions, and developmental roles. Chromosoma. PMID: 28213686
  12. Li G.,* Xu A.,* Sim S, Priest J., Tian X., Khan T., Quertermous T., Zhou B., Tsao P., Quake S., Wu S.M. (2016) Transcriptomic profiling maps anatomically patterned subpopulations among single embryonic cardiac cells. Developmental Cell. PMID: 27840109. *Equal contribution.
  13. Li G., Plonowska K., Kuppusamy R., Sturzu A., Wu S.M. (2015) Identification of cardiovascular lineage descendants at single cell resolution. Development. PMCID: PMC4352984
  14. Sharma A.*, Li G.*, Kuppusamy R.*, Hamaguchi R., Burridge P., Wu S.M. (2015) Derivation of highly purified cardiomyocytes from human induced pluripotent stem cells using small molecule-modulated differentiation and subsequent glucose starvation. J Vis Exp. PMID: 25867738. *Equal contribution.
  15. Sturzu A., Rajarajan K., Passer D., Plonowska K., Riley A., Tan T., Sharma A., Engels M., Feistritzer R., Li G., Selig M., Geissler R., Xu A., Robertson K., Scherrer-Crosbie M., Domian I., Wu S.M. (2015) Fetal Mammalian Heart Generates a Robust Compensatory Response to Cell Loss. Circulation. PMID: 25995316.
  16. Li G. *, Liu S*, Wang J, He J, Huang H, Zhang Y, Xu L. (2014) ISWI proteins participate in the genome-wide nucleosome distribution in Arabidopsis. Plant J. PMID: 24606212 *Equal contribution.
  17. Dong J, Gao Z, Liu S, Li G., Yang Z, Huang H, Xu L. (2013) SLIDE, the protein interacting domain of Imitation Switch remodelers, binds DDT-domain proteins of different subfamilies in chromatin remodeling complexes. J Integr Plant Biol. PMID: 23691993
  18. Li G. *, Zhang J*, Li J, Yang Z, Huang H, Xu L. (2012) Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis. Plant J. PMID: 22694359 *Equal contribution.